Pcdna puromycin

• liever
• Tuesday, August 1, 2023 6:36:07 PM
• 9 Comments

	File size: 3363 kB
	Views: 3696
	Downloads: 62
	Download links:
	Mirror link

Vector type. Mammalian Expression ; Selectable markers. Puromycin ; Bacterial Resistance(s). Ampicillin ; Growth Temperature. 37°C ; Growth Strain(s). Top10.Construction of pcDNA3.1-based vectors with blasticidin and puromycin resistance markers.Alt Name: pcDNA3.1+, pcDNA3.1, pcDNA™3.1(+), pcDNA 3.1 neo. Analyze: Sequence. Plasmid Type: Mammalian Expression. Promotor: CMV. Expression Level: High.pcDNA3.1/Puro-CAG-ASAP1 (Plasmid #52519) ; Selectable markers. Puromycin ; Bacterial Resistance(s). Ampicillin ; Growth Temperature. 37°C ; Growth.This pcDNA™3.1(+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker.pcDNA™3.1 (+) Mammalian Expression Vector - Thermo FisherpcDNA4-TO-Puromycin-mVenus-MAP (Plasmid #44118)Vector Database - pcDNA3.1(+) - Addgene

. Hygromycin B, Blasticidin, and Puromycin) can be used together for stable selection in. What is the difference between pcDNA™3 and pcDNA™3.1 vectors?pcDNA5FRT vector is a 5.1 kb expression vector designed for use with the Flp-In System. This vector features: 1. CMV promoter for high level expression in.Plasmid pcDNA3.1/Puro-CAG-ASAP2s from Dr. Francois St-Pierres lab contains the insert ASAP2s and is published in Elife. Selectable markers. Puromycin.Request PDF - On Jun 1, 2006, Masahiro Asada and others published Construction of pcDNA3.1-based vectors with blasticidin and puromycin resistance markers.Mammalian expression vector with the CMV promoter. The MCS is in the forward (+) orientation.51 Results for: pcdna 3.1 - Vector DatabasepcDNA3.1 Plasmid - Cell-culture DatabaseGene/Insert - Addgene. juhD453gf

The eGFP/mCherry/BFP fusion constructs were generated in the pcDNA3.1-puro expression vector after replacement of neomycin by the puromycin.. and maintained in their respective media with 1 μg/mL puromycin. shNS, nonspecific shRNA; shSIRT3, SIRT3-knockdown; pcDNA (empty.Commonly requested with: FLAG-Stx17 · pPBbsr2-4031NES · pMRXIP VPS16-GFP · pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin · pLNCX2.pcDNA-dCas9. Plasmid for expressing catalytically. Download pcDNA-dCas9.dna file. Download Plasmid Open in SnapGene. pGL3-U6-sgRNA-PGK-puromycin.pcDNA3.1-Mac-BFP2-TSERex. Plasmid. #124793. Purpose. Expresses the proton pump rhodopsin in mammalian cells under CAG promoter. Depositor.pcDNA3.1-KozATG-dL5-2XG4S-mCer3 · NFAT luciferase reporter · hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin · N-Terminal.. plasmid (ERa-EC cells) or control backbone vector pCDNA 3.1 (Invitrogen,. in selective media with 5 microgram/ml puromycin (Sigma) for 2 to 3 weeks.pcDNA3-HA-REDD1 96-110 (#189). Plasmid. #99929. Purpose. pcDNA3-HA-REDD1 111-135 (#190). Plasmid. Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc.pcDNA-dCas9-VP64. Mammalian cell plasmid for CRISPR activation by expressing. Download pcDNA-dCas9-VP64.dna file. pGL3-U6-sgRNA-PGK-puromycin.puromycin resistance gene. Use. Tags. T2A, 3xHA, and NLS. Expression. Mammalian. Mutation. Promoter. pcDNA-Ikka-HA WT. Plasmid. #23296. Depositor.Lysates of mock-treated (m), pcDNA-GFP-transfected (DNA),. After transient transfection of the CRISPR/Cas9 plasmids and 3 days of puromycin selection,.Related items: From this article · Dannel McCollum Lab Plasmids. Commonly requested with: pcDNA4-TO-Puromycin.I have constructs in pcDNA 3.1 (Invitrogen) and in pTarget (Promega). Just add puromycin and positive cells only in 1 week rolleyes.gif.pcDNA3.1+-pHlash, pRSETb-pHlash: A pH sensor created by fusing a bright mutant of Renilla luciferase (Rluc8) through an Ala-Glu-Leu linker to the circularly.Puromycin (0). Hygromycin (0). 9 Results for: pcDNA 3.1/Myc-His (-) A. pcDNA3.1(+)/myc-His A, Mammalian Expression, Neomycin, Ampicillin, Invitrogen.Catalog Number : 40257 · Product Name : pcDNA3.1/Puro-CAG-VSFP-CR · article : doi, 10.1038/nmeth.2171 · bacterial resistance : Ampicillin · cloning : backbone.Twist Bioscience synthesizes high-quality, NGS-verified custom genes at a cost and scale that are otherwise unavailable. For researchers wanting to.Plasmid pcDNA3.1(+)/Puro/FA/GFP-LL5BIP from Dr. Tomasz Prószyńskis lab contains the insert LL5BIP. Mammalian Expression. Selectable markers. Puromycin.For example, CMV-based DNA plasmids, pCR3.1 and pcDNA3.1,. For those lentiviral plasmids containing the puromycin resistance gene,.Vector type. Mammalian Expression ; Selectable markers. Puromycin ; Species. M. musculus (mouse) ; Insert Size (bp). 1743 ; Entrez Gene. Mettl3 ( a.k.a... such as pcDNA3 (Thermo Fisher Scientific, Waltham, MA, USA) [1]. The promoter is composed of a CMV promoter and IRES-puromycin.pcDNA3-P4:gs:nls:VPR. Plasmid. #135983. Purpose. Expresses the P4 coiled coil fused to the VPR transcriptional activation domain in.Puromycin, pac, HeLa, 293HEK, Jurkat T cells, Inhibits protein synthesis; premature chain termination, 1-10 ug/mL.pcDNA3.1(+)_Granulin 4-no linker. Plasmid. #176925. Purpose. pcDNA3.1(+)_Granulin 5+linker6. Plasmid. #176926. pcDNA4-TO-Puromycin-mVenus-MAP. Plasmid.Plasmids Construction. To construct pPuro-hAID plasmid, the hygromycin resistant gene in pCDNA3.1/Hygro(+) was replaced by puromycin resistant.pcDNA™3.1/myc-His A, B, and C Mam. Catalog number: V80020. 890.00 / 20 µg. pcDNA™3.1 Mammalian Expression. Catalog number: V79020. 856.00 / 20 µg.pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus. donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin.Human 293 cells were cotransfected with the pcDNA-Metnase expression vector and the plasmid, pBOS, which expresses a puromycin resistance.Plasmid, Description of plasmid. Driver plasmids. pcDNA L1-RP (37), Contains the 6.0-kb FL-L1 RP (49) cloned into pcDNA6 myc-his. pcDNA LRE4, LRE4 was made.PrecisionShuttle™ mammalian expression vector with TurboGFP and puromycin resistance markers, for fusing an ORF to C-terminal c-Myc and FLAG® tags.Mammalian IRES-containing vector with a puromycin resistance marker for expressing two genes from the same bicistronic transcript.Key Words: Expression cloning, HeLa cells, lacZ gene, pcDNA3.1/myc-His C,. and puromycin-resistance genes (23) further increasing the versatility of this.

• Pcdna addgene
• Pcdna 3.1 vector
• Pcdna vector